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Human Lung Fibroblasts

Model formats

  • Human lung fibroblasts
  • Myofibroblast transition

Cell types

  • Primary human lung fibroblasts (HLF)

Species

  • Human (Healthy donor)
  • Human (IPF donor)

Human lung fibroblast model to test the efficacy of anti-fibrotic agents in vitro

Newcells human primary lung fibroblast model is the basis of our FMT assay to evaluate anti-fibrotic therapies in vitro at high throughput. The assay replicates extracellular matrix deposition (ECM) observed in vivo when lung fibroblast injury leads to activation and proliferation. Following injury, pulmonary fibroblasts proliferate playing an important role in the repair and remodelling of tissue. However, excessive proliferation of fibroblasts can result in abnormal tissue function.

Available analytical readouts

High-throughput Imaging
  • Cell number
  • Proliferative capacity (cellular EdU incorporation)
  • Quantification of collagen I expression and deposition
  • Quantification of αSMA expression
Additional readouts
  • RNA expression changes
  • Quantification of secreted cytokines/biomarkers
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect collagen I, as a measure of extracellular matrix production and deposition.
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect collagen I, as a measure of extracellular matrix production and deposition.

Origin

Healthy and IPF donor

Assay window

72 h

Lead time

2 to 4 months

Newcells human lung fibroblast model

Accelerate your lead compound selection by understanding their mode of action in a model of lung fibrosis

1.

Evaluate therapeutic candidates targeting idiopathic pulmonary fibrosis (IPF)

2.

Easily and accurately model lung fibrosis in vitro

3.

High throughput to accelerate drug discovery

Introducing our Fibroblast-to-Myofibroblast Transition (FMT) Assay

Applications for human lung fibroblast model Close Open

Fibroblast-to-Myofibroblast Transition (FMT) assay

Human lung fibroblast model Close Open

Newcells has developed a robust and well validated model of FMT from primary lung fibroblasts isolated from healthy and IPF donors. Through stimulation of the well-established fibrogenic mediator TGF-ß, lung fibroblasts are activated and differentiate into myofibroblasts producing increased levels of extracellular matrix (Collagen I and III), which can be quantified. With this model, the ability of small molecules to reduce or increase ECM deposition can be assessed at high throughput.

Human lung fibroblast description Close Open
  1. Low passage normal human primary fibroblasts isolated from adult lung tissue
  2. Available from multiple donors including both healthy and IPF (idiopathic pulmonary fibrosis) donors
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect α-SMA, as a measure of fibroblast to myofibroblast transition.

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Fibroblasts to Myofibroblast transition
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect α-SMA, as a measure of fibroblast to myofibroblast transition.
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect collagen I, as a measure of extracellular matrix production and deposition.
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect Collagen I, as a measure of extracellular matrix production and deposition.

How to use our primary lung fibroblast model?

Outsource your experiments to us

Take advantage of our expertise: with our fast and reliable service, our in-house experts can work with you to design experiments focused on your hypothesis. Study data will present key insights into the ability of small molecules to alter ECM deposition. Our bespoke projects are carried out in our state-of-the-art facilities by our team of lung experts.

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FMT assay

We analyse your anti-fibrotic compounds, screening multiple concentrations and generating many data points at high throughput. Our service only takes 2-4 months.

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