aProximate™ Proximal Tubule Assay-Ready Plates: Advancing Drug Research with Accurate In Vitro Models
aProximate™ Proximal Tubule Assay-Ready Plates offer an innovative approach to drug research. providing an accurate in vitro model of the proximal tubule derived from fresh human kidney tissue. Unlike other primary cell models and cell lines, these unique assay-ready plates are derived from fresh human kidney tissue, never frozen or passaged, ensuring unparalleled accuracy and reliability in your experiments. With worldwide shipping options, our assay-ready plates are accessible to researchers across the globe and delivered to you ready to go.
Features and benefits
Accurately recapitulates the function of the proximal tubule
Global accessibility
Increased Efficiency with High Throughput
Understanding Species Differences
Tight junctions between aProximate™ proximal tubule cells shown by ZO-1 staining
Multiple species
Freshly isolated PTCs
Validated model
aProximate™ Assay-Ready Plates
Advancing Drug Research with In Vitro Proximal Tubule Models Delivered To Your Lab
Reliable and Accurate Results: With the use of fresh human kidney tissue, our assay-ready plates offer trustworthy results, aiding in the assessment of drug transport, metabolism, and toxicity. This reliability supports the identification of promising drug candidates and minimises potential risks.
A Realistic In Vitro Model: Emulate human physiology more realistically with our assay-ready plates, offering insights that align more closely with clinical outcomes and mechanistic understanding of renal drug handling. The aProximate™ model facilitates more precise predictions of drug behaviour and effects.
Versatile Applications: aProximate™ Assay-Ready Plates support various applications, including drug transporter studies, ADME/DMPK evaluations, and toxicity and safety screenings. This versatility streamlines experimental processes and optimises resource utilisation.
Expert Assistance: Our team of experts is committed to your success. We offer technical support and guidance to ensure that you obtain meaningful results with our assay-ready plates.
Cross Species Evaluation: We understand the importance of cross-species comparison studies in drug research. As such, we offer aProximate™ Assay-Ready Plates in rat format, allowing researchers to gain valuable insights into species-specific drug responses.
Designed with your convenience in mind, our aProximate™ Assay-Ready Plates are ready to go and enable you to focus on your research without the hassle of extensive cell culture procedures.
We ship the primary proximal tubule cells in stasis media, a gel that preserves the cells’ viability during transit. These cells are maintained at a stable 4 degrees Celsius to ensure their optimal condition upon arrival. Our streamlined delivery process ensures the cells’ integrity, and we take pride in providing high-quality products that meet your research needs.
Once the Assay-Ready Plates are in your hands, a simple and user-friendly protocol guides you through the process of removing the stasis gel and replacing it with the supplied maintenance media. This step-by-step guide ensures a smooth transition of recovery for the proximal tubule cells, allowing you to establish a functional and polarized cell monolayer with ease. All proximal tubule cells included in our assay-ready plates undergo rigorous quality control testing for your peace of mind.
TEER measurements indicate when the cells have formed a confluent monolayer, signalling their readiness for experimentation. Thus a TEER (Trans-epithelial Electrical Resistance) meter is required to determine the optimal time for assay. Our user guide provides comprehensive details on using the TEER meter effectively to assess the monolayer’s confluency.
aProximate™ is available for shipment to customers to perform kidney transporter and toxicity studies in-house. aProximate™ Assay-Ready plates are provided in either 24-well or 96-well Transwell® format.
ZO-1 Immunocytochemistry staining of PTCs (red) and Hoescht nuclear staining.
Functional validation of aProximate™ assay-ready plates for transporter assays
Functional validation studies for transcellular flux and uptake of creatinine revealed preferential basolateral-to-apical (JBA) creatinine transport, indicative of normal physiological function. A JBA uptake ratio >1.5 indicates that the cells are functional.
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