In Vitro Ciliary Beat Analysis for Lung Small Airway Epithelial Cells
The lung ciliary beat frequency analysis in vitro assay helps assess cilia function for predicting mucociliary clearance, the extent of respiratory disorders, and evaluate drug efficacy, dosing, sustained effect and safety in the lung’s small airway epithelium.
The assay uses the physiologically relevant lung SAEC cultivated under ALI culture to reliably predict in vivo results. It has been functionally validated and streamlined to accurately provide sensitive analysis of multiple functional parameters of beating cilia and can be run in conjunction with the SAEC toxicity assay to provide more data.
High-definition videos of beating ciliated cells are captured using an optimized setup designed for increased sensitivity. This setup employs Newcells’ proprietary CiliaBeat software, which quickly generates accurate datasets. These datasets provide detailed, unbiased insights into the effects of drugs on the activity of SAEC and the frequency of beating cilia, enabling confident decision-making.
Assay outputs
- Ciliary beat frequency
- % Area covered by active cilia
- Histogram of ciliated cells beating frequency distribution
- Heatmap of active ciliated cells distribution with color-coded beat frequency
